Article Details

DEVELOPMENT OF AN ACCURATE UV SPECTROSCOPIC METHOD FOR TINIDAZOLE IN VAGINAL BUFFERS

Sakshi M. Shaha , Ilyas H. Momina , Riya M. Patela and Shreeraj H. Shaha *

a Department of Pharmaceutical Technology, L. J. Institute of Pharmacy, L. J. University, L. J. Campus, Near Sarkhej-Sanand Circle, Off. S.G. Road, Ahmedabad-382 210, Gujarat, India

For Correspondence: E-mail: shreeraj.shah@ljinstitutes.edu.i 

 

https://doi.org/10.53879/id.61.05.14405


ABSTRACT

A precise UV spectroscopic method was developed for accurately quantifying tinidazole (TNZ) using the Shimadzu 1900i UV spectrophotometer. Different sample solutions containing TNZ were scanned across a range of concentration 6 µg mL-1 to 26 µg mL-1 between 200-400 nm, generating overlay TNZ spectra showing an absorption maximum at 318nm in vaginal buffer which is made up of phosphate buffer (PB) 4.2 pH with 2% Tween 80® LR and 4 mL of methanol. The construction of a six-point calibration curve demonstrated the linearity of TNZ within the 6-26 µg mL-1 concentration range. The regression equation derived from this calibration curve was subsequently employed to accurately determine the concentration in accuracy studies. In the analysis of bulk TNZ, a recovery rate ranging from 98.41% to 102.28% was achieved. The analysis results were validated following ICH (International Council on Harmonization) guidelines and compiled with the required criteria and standards

Year 2024 | Volume No. 61 | Issue No.5 | Page No. 64-67
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