Article Details

SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF SULPHACETAMIDE SODIUM AND PREDNISOLONE ACETATE IN OPHTHALMIC FORMULATION

Satish A. Patela and Dharmendrasinh A. Bariab*

a Department of Pharmaceutical Chemistry and Quality Assurance, Shree S. K. Patel College of Pharmaceutical Education & Research, Ganpat University, Ganpat Vidyanagar – 384 012, Mehsana, North Gujarat, India

b Department of Pharmaceutical Chemistry, A. R. College of Pharmacy and G. H. Patel Institute of Pharmacy, Vallabh Vidyanagar-388 120, Anand, Gujarat, India

For Correspondence: E-mail: paji5785@gmail.com

 

https://doi.org/10.53879/id.60.02.12594


ABSTRACT

A simple, precise, accurate, and rapid derivative spectrophotometric approach for the simultaneous quantification of sulfacetamide sodium (SUL) and prednisolone acetate (PRE) in the combined dosage form has been established and validated. The wavelengths selected for analysis are 230 nm (ZCP of SUL) and 244 nm (ZCP of PRE) for SUL and PRE, respectively. The suggested technique has exhibited linearity for a concentration range of 2–6 µg mL-1 for PRE and 5–15 µg mL-1 for SUL, respectively. Both medications had percentage recoveries that varied from 99.41 to 101.8. The suggested approach has been validated according to ICH guidelines [Q2(R1)] for linearity, LOD, LOQ, accuracy and precision. The proposed method can be successfully utilized to analyze SUL and PRE concurrently in ocular formulations.

Year 2023 | Volume No. 60 | Issue No.2 | Page No. 83-85
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