ABSTRACT

A simple, precise, accurate, and rapid derivative spectrophotometric approach for the simultaneous quantification of sulfacetamide sodium (SUL) and prednisolone acetate (PRE) in the combined dosage form has been established and validated. The wavelengths selected for analysis are 230 nm (ZCP of SUL) and 244 nm (ZCP of PRE) for SUL and PRE, respectively. The suggested technique has exhibited linearity for a concentration range of 2–6 µg mL-1 for PRE and 5–15 µg mL-1 for SUL, respectively. Both medications had percentage recoveries that varied from 99.41 to 101.8. The suggested approach has been validated according to ICH guidelines [Q2(R1)] for linearity, LOD, LOQ, accuracy and precision. The proposed method can be successfully utilized to analyze SUL and PRE concurrently in ocular formulations.