a Department of Quality Assurance, Ali Allana College of Pharmacy, Akkalkuwa, Nandurbar-425 415, Maharashtra, India
b Department of Quality Assurance, Jamia college of Pharmacy, Akkalkuwa, Nandurbar-425 415, Maharashtra, India
c Department of Pharmaceutics, Care College of Pharmacy, Warangal Rural, Oglapur - 506 006, Telangana, India
d Department of Pharmaceutical Chemistry, College of Pharmacy, King Khalid University, Abha-61421, Saudi Arabia
e Department of Pharmacology, Anwarul Uloom College of Pharmacy, Mallepally, Hyderabad-500 001, Telangana, India
* For Correspondence: E-mail: amshirman@gmail.com
https://doi.org/10.53879/id.59.01.12445.
ABSTRACT
This experiment aims to develop and validate the RP-HPLC method for clofarabine in a bulk and pharmaceutical dosage form. Chromatographic separation was achieved on Inertsil ODS 3V (150× 4.6 mn) diameter column. The mobile phase comprised of phosphate buffer (pH 4.0): methanol (40:60) at a flow rate of 1 mL min-1 and all eluents were detected at 270 nm. The runtime was 10 minutes. Calibration curves at five levels for clofarabine were linear in the range of 10-30 µg mL-1. Accuracy for clofarabine was studied in the range of 50-150 % quality control standard levels. A validated method was found to be accurate, reproducible, linear, precise, and robust.
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