Articles Accepted

ABSTRACT Present study highlights effectiveness of integrated analytical strategy using ultra-performance liquid chromatography with photodiode array and Quadrupole Dalton-based multi-attribute method for quantification and rapid identification of Desmopressin acetate, a model peptide drug and its degradation products, minimizing requirement for High Resolution Mass Spectrometer (HRMS) instrumentation for peptide analysis. Separation was performed on Inertsil ODS 3V (250 × 4.6 mm, 5 μm) column at 220 nm using water–formic acid–acetonitrile gradient (0.50 mL min-1, 30 °C). A microdroplet thermal platform using the Leidenfrost effect enabled low volume forced degradation studies under acidic, basic, and oxidative conditions. Deamidation of glutamine and asparagine dominated, with additional oxidative and base induced pathways, yielding total ten degradation products across both studies whose structures were proposed based on mass data and mechanistic rationale. Method validation complied with ICH Q2(R2), demonstrating specificity, linearity (R² = 0.9985), accuracy 98.1% (93.9–100.5%), precision 100.4% (0.51% RSD), robustness, and solution stability.