Articles Accepted

Background: This study investigates the development of a phytosomal gel loaded with Mangiferin fraction to improve the bioavailability and therapeutic efficacy of the plant's wound-healing constituents through phytosomal encapsulation. Methods: Mangiferin fraction was encapsulated into phytosomes using lecithin. These phytosomes were then incorporated into a gel matrix to formulate the final phytosomal gel. Spreadability and viscosity were analyzed to assess the impact of formulation variables, with regression analysis determining the effects of Carbopol® 934P and PEG 400 concentrations. Particle size distribution and zeta potential measurements were conducted to ascertain the colloidal stability of the phytosomes. The wound healing efficacy was evaluated using an In-vivo model with Balb-c mice, and a stability study was performed under accelerated conditions. Results: The formulated phytosomal gel demonstrated a particle size of 171.2 nm (±39.2 nm) and a zeta potential of -35.4 mV (±4.11 mV), indicating a stable and homogenous system. No drug-excipient interactions were detected in the DSC analysis. After two months of storage at stressed conditions, the optimized formulation (F6) maintained its stability, with minor changes in viscosity and spreadability. The in vivo model showed that the phytosomal gel significantly enhanced wound healing by day 10 compared to the control group. Conclusion: The phytosomal gel containing Mangiferin fraction displayed promising wound healing potential with a stable and effective delivery system. The positive outcomes of the In-vivo studies suggest that this phytosomal formulation could be a valuable addition to wound care treatments.