Articles Accepted

A simple precise and accurate stability indicating Ultraviolet spectroscopic method was developed and validated for simultaneous estimation of azelnidipine and chlorthalidone in bulk and pharmaceutical dosage form by Absorbance- correction method. Ethanol is used as the solvent. For chlorthalidone, the detection wavelength was found to be 275 nm and for azelnidipine 345 nm. The developed method was validated in terms of sensitivity, linearity, reproducibility, accuracy, robustness and ruggedness. Beer-lamberts law was obeyed at concentration ranging from 3.2–80 µgmL-1 for azelnidipine and 5-125 µgmL-1 for chlorthalidone. The limit of detection was found to be 1.74 µgmL-1 for azelnidipine and 2.376 µgmL-1 for chlorthalidone. The limit of quantification was found to be 5.272 µgmL-1 for azelnidipine and 7.2 µgmL-1 for chlorthalidone. The reproducibility and repeatability outcome were all within the limits. Accelerated stability studies were carried out. Azelnidipine and chlorthalidone showed different degradation characteristics under acid, alkali, humidity, heat, and oxidized environment.