There is a huge interest in medicinally active constituents isolated from plants. The β-Amyrin is a class of pentacyclic triterpenoids and it is oleanane substituted at the 3 beta-position by a hydroxyl group and containing a double bond between positions 12 and 13. These triterpenoids are generally found in many medicinal plants. The β-Amyrin is generally extracted and isolated from leaves, oleoresin (latex) exudates by the bark incisions of the plant sources from many plant species. Earlier reported β-amyrin isolation through column chromatography but in this article, we developed a novel method for isolation. Triterpenoids are constituents that have had a great interest in recent years due to their pharmacological potential, with numerous therapeutic activities, such as anticancer, anti-inflammatory, antiviral, antibacterial, antifungal, ant-diuretic, and acetylcholinesterase inhibitors. The isolated beta amyrin is characterized and confirmed by all analytical methods. (Melting point, TLC, IR, MASS and NMR).