Articles Accepted

Development and validation of Reverse Phase High Performance Liquid Chromatography method for simultaneous estimation of Rosuvastatin and Teneligliptin in their synthetic mixture
by Ms. Minakshi M. Dhoru, 28 May 2021
Co-Author(s): Maitri Parikh,Krunal Detholia,Pathik Patel

A simple, rapid and precise Reverse Phase High Performance Liquid Chromatography method has been developed and validated for estimation of rosuvastatin and teneligliptin in their synthetic mixture. The chromatographic separation was achieved on Agilent Inertsil Octa Decyl Silane (ODS) C18 (250 × 4.6 mm, 5 µm) column using mobile phase composition of Methanol: Water (pH=3 adjust with 10% Orthophosphoric acid) (70:30 %v/v), in isocratic mode at flow rate of 0.8 mL/min. at a detection wavelength of 244nm. The method was validated in terms of precision, accuracy, limit of detection and limit of quantitation. Retention time for rosuvastatin and teneligliptin was found to be 7.41 min and 4.35 min respectively. Linearity of rosuvastatin and teneligliptin was found to be 5-15 µg/mL and 10-30 µg/mL respectively with correlation coefficient (R2) value of 0.998 for rosuvastatin and 0.999 for teneligliptin. Method can be employed for analysis of rosuvastatin and teneligliptin in their mixture.

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August 2022
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