Article Details

HPTLC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTIFICATION OF ALECTINIB HYDROCHLORIDE IN BULK POWDER AND CAPSULE PREPARATIONS

Shaileshkumar K. Koradiaa *, Rushik P. Thakkara , Ashok N. Mahajana , Falgun A. Mehtaa , Priyal R. Patela and Digvijaysinh G. Ranaa

a Department of Pharmaceutical Chemistry & Quality Assurance, Krishna School of Pharmacy & Research, Drs. Kiran & Pallavi Patel Global University, Krishna Educational Campus, Vadodara – Mumbai NH # 8, Varnama, Vadodara – 391 243, Gujarat, India

*For Correspondence: E-mail: shaileshkoradia.ksp@kpgu.ac.in

 

https://doi.org/10.53879/id.60.10.13519


ABSTRACT

The high performance thin layer chromatography method described here enables simple, accurate and precise estimation of alectinib hydrochloride content in capsule formulation. To achieve adequate separation, G60 - F254 pre-coated silica gel sheets of aluminium were used as the stationary phase. A mobile phase comprising a solvent mixture of methanol and water in a ratio of 80:20 (V/V) was utilized with a chamber saturation duration of 30 minutes. The retardation factor value was found to be 0.47 at the detection wavelength 341 nm. The method’s linearity was confirmed across a concentration range from 600 - 2100 ng band-1, exhibiting a high correlation coefficient of 0.9992. The accuracy range was observed between 98.50 % – 100.45 %. The lowest detection limits of drug were observed to be 17.49 ng band-1 and lowest quantitation limits of drug were found to be 53.01 ng band-1. This developed methodology was effectively employed for the quantification of alectinib hydrochloride in capsule preparations

Year 2023 | Volume No. 60 | Issue No.10 | Page No. 53-57
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