Article Details

HPTLC AND FIRST DERIVATIVE UV-SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF VILAZODONE IN PHARMACEUTICALS

Sagar S. Pandaa * and Ravi Kumar V.V. Beraa

a Department of Pharmaceutical Analysis & Quality Assurance, Roland Institute of Pharmaceutical Sciences (Affiliated to Biju Patnaik University of Technology, Odisha), Khodasingi, Berhampur-760 010, Odisha, India

*For Correspondence: E-mail: sagarguddu2002@gmail.com

 

https://doi.org/10.53879/id.60.01.12718


ABSTRACT

A new HPTLC method and a sensitive UV spectrophotometric method were established for estimating vilazodone hydrochloride in its pharmaceutical formulations. An environment-friendly chromatographic mobile phase consisting of ethyl acetate: toluene: formic acid (5:4:1, V/V/V) along with ultraviolet densitometric detection at 240 nm using pre-coated silica gel plates was used. First derivative UV spectrophotometric determinations were performed using methanol as solvent, and amplitudes were measured for quantification purposes. Validation studies were compliant with ICH guidance. Vilazodone shows an optimum Rf value of 0.73 ± 0.02 by HPTLC method. First derivative spectrophotometric measurements were done at 230 nm (peak) and 247 nm (valley), respectively. Best method linearity was observed in the range of 100 - 600 ng band-1 and 100 - 600 ng mL-1 for the chromatographic and spectrophotometric methods, respectively. Method detection limits were 61.4 ng band-1 and 36.03 ng mL-1, for chromatography and spectrophotometry, respectively. Higher % recovery (> 99 %) of vilazodone from the in-house formulation satisfied study intent as well as instituted selectivity of both the methods. Both the newly developed methods have the potential of application in routine quantification of vilazodone in pharmaceuticals.

Year 2023 | Volume No. 60 | Issue No.1 | Page No. 61-66
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