Articles Accepted

Advancing Parkinson's Disease Detection with FLISA: A Novel Approach Targeting Misfolded α-Synuclein
by Dr. Sadhana Sathaye, 03 Oct 2023
Co-Author(s): Paramita Batabyal,Vishwas Sarangdhar,Ajay S Sawner,Amruta V Gadade,Radni D Deshpande,Vikas Dighe

A Fluorescence-Linked Immunosorbent Assay (FLISA) targeting β-cross-sheet α-synuclein protein, a crucial marker in Parkinson's Disease (PD), was assessed using in-vitro assays with a rotenone-induced PD cellular model. The study aimed to evaluate FLISA's applicability with the in-house developed labelled polyclonal antibody (pAb). Results are promising; the competitive assay successfully distinguished misfolded α-synuclein in SH-SY5Y cells. Quantitative analysis revealed a direct correlation between competitive antigen concentration and decreased fluorescence, showcasing FLISA's sensitivity. Controls exhibited maximal fluorescence, confirming the absence of misfolded proteins, while rotenone-exposed cells displayed reduced fluorescence, suggesting their presence. This approach enhances PD understanding and supports potential interventions.

Current Issue
December 2023
Quick Contact