Articles Accepted

Background: Literature survey of Ethambutol reveals that no stability indicating analytical method is reported for its estimation eitherin bulk or in pharmaceutical dosage form. Objective: It was therefore found a need for developing and validating stability indicating analytical method to estimate Ethambutol by RP-HPLC. Method: In the present study, a novel analytical method is developed for separating Ethamubutol from its degradation products. The degradation products are formed by exposing the drug to various stress conditions like hydrolysis, oxidation, neutral and photolysis decompositionand performing their separation using mobile phase buffer: acetonitrile (90: 10 v/v) using ODS Hypersil C-18 (250mm ×4.6mm, 5.0µm) column, flow rate of 0.5 mL/min with UV detection at 210 nm. Results: Retention time for Ethambutol was found to be 6.1 min. The validation of the method was performed as per ICH guidelines. Conclusion: The developed method can be referred by pharmaceutical industries for performing routine analysis of drug in pharmaceutical dosage forms.