ABSTRACT

Double Divisor ratio spectra derivative method was developed and validated for a ternary mixture containing abacavir sulphate (ABA), lamivudine (LAM) and dolutegravir sodium (DOL) in distilled water. The method is based on dividing the absorption spectra of ternary mixture by the sum of absorption spectra of two of the three components and then taking the first derivative of the ratio spectra. The linear range was found to be 5 – 40 µg mL^-1 for ABA, 4 – 20 µg mL^-1 for LAM and 1 - 20 µg mL^-1 for DOL. The mean % recoveries were found to be in the range of 98.85 – 100.93 % for ABA, 99.65 – 99.38 % for LAM and 100.29 – 98.67 % for DOL. ICH guidelines were used to validate the developed method. The proposed method can be successfully applied to pharmaceutical formulations as it is rapid, simple, sensitive, selective and accurate.