Articles Accepted

Extraction, Identification and Estimation of Caffeine in Green and Black Tea samples by a Simple UV-Visible Spectroscopic Method
by Dr. Archana, 05 Jul 2021
Co-Author(s): Ravali M

Caffeine 1, 3, 7-trimethylxanthine is a widely consumed psychoactive substance worldwide. It is a diuretic and CNS stimulant. Apart from stimulating the central nervous system, caffeine exerts positive effects in the body, often in association with other substances like L Theanine and Catechins. Caffeine was extracted from various solvents like Ethyl acetate, Dichloromethane and Acetone. The yield of caffeine from green tea in Dichloromethane, Ethyl acetate and Acetone extracts was found to be 0.85%, 0.72%, 0.81% respectively. The Yield of caffeine from Black tea in Dichloromethane, Ethyl acetate and Acetone was found to be 1%, 0.82% and 0.95% respectively. The maximum yield of caffeine was obtained from Dichloromethane. The extracted caffeine was identified by TLC and FTIR. A simple UV spectroscopic method was developed and validated for the estimation of caffeine in Green tea and Black tea. The Maximum Wavelength of caffeine obtained at 272nm in distilled water. The Beers law obeyed in the concentration range of 0.5 to 35ug/ml for caffeine. This method was validated with respect to linearity, accuracy, precision, robustness, and ruggedness, limit of detection and limit of quantification as per ICH guidelines. Percentage recovery was 96.03 to 96.23%. LOD and LOQ values were 0.027 ug/ml and 0.082ug/ml respectively. The percentage Purity of Caffeine from Green tea and Black tea was found to be 96.64% and 94.49%. This method was found to be precise as % RSD was less than 2. Thus, the proposed method was found to be rapid, specific, precise and accurate for the routine analysis of Caffeine in Green and Black tea samples.

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