Articles Accepted

HPTLC AND FIRST DERIVATIVE UV-SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF VILAZODONE IN PHARMACEUTICALS
by Mr. SAGAR SUMAN PANDA, 09 Sep 2020
Co-Author(s): Ravi Kumar Venkata Varaha Bera

A new HPTLC method and a sensitive UV spectrophotometric method were established for estimating vilazodone hydrochloride in its pharmaceutical formulations. An environment-friendly chromatographic mobile phase consisting of ethyl acetate: toluene: formic acid (5:4:1, v/v/v) along with ultraviolet densitometric detection at 240nm using pre-coated silica gel plates was used. First derivative UV spectrophotometric determinations were performed using methanol as solvent, and amplitudes were measured for quantification purposes. Validation studies were compliant with ICH guidance. Vilazodone shows an optimum Rf value of 0.73±0.02 by HPTLC method. First derivative spectrophotometric measurements were done at 230nm (peak) and 247nm (valley), respectively. Best method linearity was observed in the range of 100-600ng/band and 100-600ng/mL for the chromatographic and spectrophotometric methods, respectively. Method detection limits were 61.4ng/band and 36.03ng/mL, for chromatography and spectrophotometry, respectively. Higher % recovery (>99%) of vilazodone from the in-house formulation satisfied study intent as well as instituted selectivity of both the methods. Both the newly developed methods have the potential of application in routine quantification of vilazodone in pharmaceuticals.

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